Kappa Casein Gene Polymorphism in Holstein
Chinese Cattle
A. E. Hamza1*, X. L. Wang and Z. P.
Yang
Animal Science and Technology College, Yangzhou
University, Yangzhou, China; 1Department of Animal
Production, College of Veterinary Science, Nyala University, Nyala-Sudan.
*Corresponding author:
hamzab2007@hotmail.com;
hamzab2003@yahoo.com
Abstract
Kappa casein gene polymorphism has received a
considerable attention because of its correlation with milk quality, composition
and technological properties. The polymorphism of kappa casein gene (K-CN) was
detected in Holstein Chinese cattle. A 218 bp sequence in exon IV of 319
Holstein Chinese cattle blood samples were amplified using polymerase chain
reaction-single strand conformation (PCR-SSCP) technique. Sequence analysis
revealed one single nucleotide polymorphism (SNP) T/C SNP in exon 1V at
nucleotides (80), moreover; three genotypes TT, TC and CC were also identified
with following frequencies: 0.40, 0.34 and 0.26%, respectively. The allele
frequency for T and C found to be 0.6 and 0.4 %, respectively. Allele
frequencies in the population fitted with Hardy–Weinberg equilibrium (P >
0.05). Analysis of genetic polymorphism of k-casein at exon 1V exhibited medium
polymorphism information content (PIC=0.36).
Key words: Holstein
Chinese cattle,
Kappa casein gene Polymorphism, PCR-SSCP, Single nucleotide