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Short Communication
Comparison of Photometer with Improved Neubauer Hemocytometer and Makler Counting Chamber for Sperm Concentration Measurement in Cattle
Naima Atiq, Nemat Ullah1*, S. M. H. Andrabi2 and Shamim Akhter
Department of Biological Sciences; 1Faculty of Veterinary and Animal Sciences, PMAS-Arid Agriculture University, Rawalpindi; 2Animal Reproduction Programme, Animal Sciences Institute, National Agricultural Research Center, Islamabad, Pakistan
*Corresponding author:


The present study was designed to compare the photometer (Lp 300 SDM Minitüb GmHb) with improved Neubauer hemocytometer and Makler counting chamber for sperm concentration measurement in cow bulls. Data were based on 35 cow bull semen samples. The average sperm concentrations (109/ml) determined by photometer, hemocytometer and Makler chamber were 1.35±0.72, 1.17±0.53 and 1.49±0.60, respectively. Analysis of variance revealed that there was no difference among the three techniques of sperm concentration measurement of same semen samples in cow bulls. It was concluded that the use of photometer in semen evaluation for sperm concentration reduced chances of human error and time consumption effectively.

Key words: Hemocytometer; Makler chamber; Photometer; Sperm concentration


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)