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Indirect Fluorescent Antibody Technique based Prevalence of Surra in Equines
Ahsan Nadeem, Asim Aslam*, Zafar Iqbal Chaudhary, Kamran Ashraf1, Khalid Saeed1, Nisar Ahmad1, Ishtiaq Ahmed and Habib ur Rehman2
Department of Pathology, 1Department of Parasitology, Department of Physiology2, University of Veterinary & Animal Sciences, Lahore, Pakistan
*Corresponding author:


This project was carried out to find the prevalence of trypanosomiasis in equine in District Gujranwala by using indirect fluorescent antibody technique and thin smear method. Blood samples were collected from a total of 200 horses and donkeys of different ages and either sex.  Duplicate thin blood smears were prepared from each sample and remaining blood samples were centrifuged to separate the serum. Smears from each animal were processed for giemsa staining and indirect fluorescent antibody test (IFAT). Giemsa stained smears revealed Trypanosome infection in 4/200 (2.0%) samples and IFAT in 12/200 (6.0%) animals.

Key words: Equines; Indirect fluorescent antibody technique; Trypanosomiasis


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)