The In
vitro Antibacterial Activity of Florfenicol in Combination with
Amoxicillin or Cefuroxime against Pathogenic Bacteria of Animal
Origin
Myung-Jin Choia,
Eun-Mi Leea, Seung-Jin Lee, Md. Ahsanur Reza, Joong-Su Lee,
Elias Gebru, Man-Hee Rhee and Seung-ChunPark*
Laboratory of
Veterinary Pharmacokinetics & Pharmacodynamics, College of
Veterinary Medicine, Kyungpook National University, Daegu 702-701,
Korea *Corresponding author: parksch@knu.ac.kr
Abstract
This study evaluated the in vitro activity of florfenicol
(F) in combination with amoxicillin (AM) or cefuroxime (CRM) against pathogenic
bacteria of animal origin, including E.
coli, S. aureus,S. cholerasuis and
P. mirabilis. The MIC of AM ranged from 16 to 256 μg/ml. The MBC of
AM (64 μg/ml) was four-fold higher than its MIC value (16 μg/ml) for
E. coli, and similar to the MIC for
the other three species. The MIC of F ranged from 8 to 16 μg/ml. The MBC values
of F for E. coli,
S. aureus, and S. cholerasuis
were eight-fold higher than the respective MIC values, and 32-fold higher
than the MIC of P. mirabilis. The MIC
of CRM ranged from 8 to 128 μg/ml. The MBC of CRM was the highest (³ 256 μg/ml),
except for E. coli. The F/AM
combination resulted in synergism (FIC index £
0.5) for E. coli, S. aureus, and
P. mirabilis and in-difference (FIC
index >1) for S. cholerasuis. For
F/CRM combination, synergism (E. coli
and S. cholerasuis)and in-difference (S. aureus and P. mirabilis)
were observed. Killing rate study showed a 1.5 - > 3 log 10 cfu/ml reduction of
E. coli with F/AM compared to AM or F
alone. The highest activity of the combinations was observed when F comprised at
least 50% of the combination. Further studies using many bacterial isolates and
various proportion of each drug would reveal the potential of a combination
product containing F and AM/CRM for use in veterinary practice.