College of
Animal Science
and Veterinary Medicine, Shandong Agricultural University, Daizong
Road No. 61, Taian, Shandong 271018, China;
1Shandong
Key Laboratory of Animal Disease Control and Breeding, Institute of
Animal Science and Veterinary Medicine, Shandong Academy of
Agricultural Sciences, Sangyuan Road No.10, Jinan 250100, China
*Corresponding author:
sdauyan@sdau.edu.cn;
zhurl@sdau.edu.cn
Abstract
To evaluate mucosal immunity of normal and immunosuppressed chickens vaccinated
with live
Newcastle
disease
(ND)
vaccine, cyclophosphamide (CY) was used to generate immunosuppressed chickens.
Normal
and immunosuppressed chickens were vaccinated with the Lasota ND vaccine by
ocular-nasal route at three weeks of age and challenged with
virulent ND virus (vNDV) at day 28 post-vaccination (pv).
The immunosuppressed chickens had significantly lower relative weight of the
bursa of Fabricius and serum antibody HI titers compared to CY-untreated
chickens. Compared with normal chickens, significant lower levels of
IgA antibodies were detected in
tracheal washings, duodenal washings and bile of immunosuppressed chickens
in the whole experimental period.
Immunohistochemical experiment also showed that small numbers of
IgA positive cells
were found in intestinal tissues
of immunosuppressed chickens
at day 28 pv.
There was only a partial protective effect on immunosuppressed chickens post
challenge with
virulent ND virus (vNDV).
These findings increase our understanding of the protective mucosal immune
response against ND vaccine and suggest that
mucosal immunity play an important role against NDV infection.