Changing Trend of T lymphocytes in Mouse Spleen after Japanese
Encephalitis Virus Infection
Yan-fang Sun§, Chang-qin Gu§,
Rong Jiang, Jing Ye, Yong-mao Li, Hua-zhen Liu, Hui Songand
Ke-mei Peng*
College of Veterinary
Medicine, Huazhong Agricultural University, Wuhan 430070, PR China *Corresponding author: kmpeng@sohu.com;
pengkm@mail.hzau.edu.cn
Abstract
Japanese encephalitis is caused by Japanese
encephalitis viruses (JEV) with neurotropism. As one of the most important
immune organ, spleen is directly involved in immune response against JEV.
However, little research about JEV infection process in spleen has been
reported. In this study, immunopathological changes in mouse spleen were
analyzed every other day
after subcutaneous injection of mice with JEV wild-type strain
P3 by immunohistochemistry assay.
Immunohistochemistry analysis demonstrated that the number of T lymphocytes was
reduced from 0 to 3 DPI, increased from3 to 7 DPI, and reduced again from
7 to 10 DPI. In addition, neurological dysfunction appeared at 6 DPI. These results suggested
that spleen of mice suffered incontrovertible damages in influence of JEV
infection. It can also be deduced that the cellular immunity took the crucial
part in the first phase of transient viremia against JEV. Moreover, immune response was activated after
the immune-depressed period in the first phase of viremia and neurological dysfunction appeared when
cellular immunity was activated. Taking together, our research showed distinct immunopathological changes in mice after JEV
infection, which enriched our understanding of Japanese encephalitis
immunopathogenesis.
Key words: Immunohistochemistry;
Japanese encephalitis virus;
Mice;
Spleen; T lymphocytes