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Pak Vet J, 2012,
32(1):
134-135
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Detection
of
Porcine Circovirus Type 2 (PCV2)
in
Mosquitoes from Pig
Farms
by
PCR |
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Xiaohong Yang, Lidan Hou, Jing Ye, Qigai He and
Shengbo Cao |
State Key Laboratory
of Agricultural Microbiology and Laboratory of Animal Virology,
College of Veterinary Medicine Huazhong Agricultural University, Wuhan, Hubei,
430070, P. R. China
*Corresponding author:
sbcao@mail.hzau.edu.cn |
Abstract
To investigate the whether mosquitoes could be
potential vectors that maintain or transmit PCV2, 59 mosquito samples were
collected from suspicious PCV2-infected pig farms in Hubei
and Anhui province in China.
Total DNA of these mosquitoes were extracted and then
tested for
presence of PCV2
nucleic acid by PCR. Four (6.78%) samples showed the positive result.
Subsequently, the positive PCR product was cloned into
pEASY-T1 vector and sequenced. Sequence analysis
displayed that homology between the PCR products and PCV2 strains were more than
94%. These results demonstrate that PCV2 nucleic acid exists in these mosquitoes, which suggests that mosquitoes could serve as
mechanical transmission vectors of PCV2.
Key words:
Mosquitoes,
PCV2,
Polymerase chain reaction (PCR),
Vector
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ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)
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