Detection of
Porcine Circovirus Type 2 (PCV2) in
Mosquitoes from Pig
FarmsbyPCR
Xiaohong Yang, Lidan Hou, Jing Ye, Qigai He and
Shengbo Cao
State Key Laboratory
of Agricultural Microbiology and Laboratory of Animal Virology,
College of Veterinary Medicine Huazhong Agricultural University, Wuhan, Hubei,
430070, P. R. China *Corresponding author:
sbcao@mail.hzau.edu.cn
Abstract
To investigate the whether mosquitoes could be
potential vectors that maintain or transmit PCV2, 59 mosquito samples were
collected from suspicious PCV2-infected pig farms in Hubei
and Anhui province in China.
Total DNA of these mosquitoes were extracted and then
tested for presence of PCV2
nucleic acid by PCR. Four (6.78%) samples showed the positive result. Subsequently, the positive PCR product was cloned into
pEASY-T1 vector and sequenced. Sequence analysis
displayed that homology between the PCR products and PCV2 strains were more than
94%. These results demonstrate that PCV2 nucleic acid exists in these mosquitoes, which suggests that mosquitoes could serve as
mechanical transmission vectors of PCV2.