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Detection of Porcine Circovirus Type 2 (PCV2) in Mosquitoes from Pig Farms by PCR
Xiaohong Yang, Lidan Hou, Jing Ye, Qigai He and Shengbo Cao
State Key Laboratory of Agricultural Microbiology and Laboratory of Animal Virology, College of Veterinary Medicine Huazhong Agricultural University, Wuhan, Hubei, 430070, P. R. China
*Corresponding author:


To investigate the whether mosquitoes could be potential vectors that maintain or transmit PCV2, 59 mosquito samples were collected from suspicious PCV2-infected pig farms in Hubei and Anhui province in China. Total DNA of these mosquitoes were extracted and then tested for presence of PCV2 nucleic acid by PCR. Four (6.78%) samples showed the positive result. Subsequently, the positive PCR product was cloned into pEASY-T1 vector and sequenced. Sequence analysis displayed that homology between the PCR products and PCV2 strains were more than 94%. These results demonstrate that PCV2 nucleic acid exists in these mosquitoes, which suggests that mosquitoes could serve as mechanical transmission vectors of PCV2.

Key words: Mosquitoes, PCV2, Polymerase chain reaction (PCR), Vector


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)