Effect of Orally Administered
Enterococcus faecium EF1 on Intestinal Cytokines and
Chemokines
Production of Suckling Piglets
Yi Huang§, Ya-li Li, Qin Huang, Zhi-wen Cui, Dong-you Yu,
Imran Rashid Rajput, Cai-hong Hu and Wei-fen Li*
Key Laboratory of Molecular Animal Nutrition and Feed Sciences,
College of Animal Science, Zhejiang University, Hangzhou,
P.R. China,
310058;
§Present
address: College of Animal Science and Technology, Guangxi
University, Nanning,
P.R. China,
530005
*Corresponding author:
wfli@zju.edu.cn
Abstract
The objective of this study was to determine the effect of orally administered
Enterococcus faecium EF1 on
intestinal cytokines and chemokines production in piglets.
Twenty-four newborn piglets were randomly divided into two groups. The treatment
group (T1), orally
administered sterilized (110 ºC for 30 min) skim milk 10% (2
ml/piglet/day) with addition of viable E.
faecium EF1 (5~6×108 cfu/ml) on 1st, 3rd
and 5th day after birth. The control group (T0), were fed
the same volume of sterilized skim milk without addition of probiotics. Feeding
trial was conducted for 25 days of suckling age. At the end of trail six piglets
were randomly selected from each group to collect the samples of jejunum and
ileum mucosa to observe the cytokines and chemokines production. The results
showed that concentrations of IL-10 and TGF-β1 significantly increased in T1
group. Whereas, production of IL-1β, IL-6, IL-12, IFN-γ and IL-8 decreased in T1
compared to T0. Levels of TNF-α were increased in jejunal mucosa,
while decreased in ileal mucosa comparatively in T1 group. Our
findings revealed that oral administration of
E. faecium EF1 induced a strong anti-inflammatory response in the small
intestine. These immunomodulatory effects of this bacterium might contribute to
maintenance of immune homeostasis in the intestine of piglets.