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Identification and Antibiotic Sensitivity of the Causative Organisms of Sub-clinical Mastitis in Goats and Sheep
 
Mohammad Rafiqul Islam1, 5, Md. Sabbir Ahamed2, Md. Shahin Alam1, Md. Masudur Rahman3, Tazminaz Sultana4, Yoon-Seok Roh5 and Bumseok Kim5*
1Goat and Sheep Production Research Division, Bangladesh Livestock Research Institute, Dhaka, Bangladesh; 2Biotechnology and Genetic Engineering Discipline, Life Science School, Khulna University, Khulna, Bangladesh; 3Department of Pathology and Parasitology, Sylhet Agricultural University, Sylhet, Bangladesh; 4Department of Zoology, Khilgaon Model College, National University, Dhaka, Bangladesh; 5Laboratory of Pathology, College of Veterinary Medicine, Chonbuk National University, Jeonju, South Korea.
*Corresponding author: bskims@jbnu.ac.kr (B. Kim)

Abstract   

This study was conducted to isolate and identify organisms responsible for sub-clinical mastitis (SCM) in goats and sheep for the determination of point prevalence of SCM and antibiotic sensitivity of the identified organisms. For this purpose 50 each of lactating sheep and goats were examined with the commercially available Leucocytest® SCM detection kit. It was found that 4 and 36% sheep and goats suffered from SCM, respective. The prevalence of clinical mastitis (CM) was 4 and 6% in sheep and goats, respectively. Milk samples were collected individually from sheep and goats with SCM and were cultured in different media including nutrient agar, blood agar and eosin methylene blue agar. The bacteria were further characterized by biochemical tests. In both goats and sheep, the organisms responsible for SCM were Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). The organisms were found most sensitive to gentamicin (Gn). After Gn treatment to goats and sheep with SCM, total bacterial counts decreased and milk production significantly increased compared to levels prior to treatment. It is suggested from the study that early detection of SCM and treatment with proper antibiotics can control SCM in goats and sheep.

Key words: Antibiotic sensitivity; Bacteria;Goats; Sheep; Sub-clinical mastitis

 
   

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)



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