Identification and Antibiotic Sensitivity of the Causative Organisms
of Sub-clinical Mastitis in Goats and Sheep
Mohammad Rafiqul Islam1, 5, Md. Sabbir Ahamed2,
Md. Shahin Alam1, Md. Masudur Rahman3,
Tazminaz Sultana4, Yoon-Seok Roh5
and Bumseok Kim5*
1Goat
and Sheep Production Research Division, Bangladesh Livestock
Research Institute, Dhaka, Bangladesh; 2Biotechnology and
Genetic Engineering Discipline, Life Science School, Khulna
University, Khulna, Bangladesh; 3Department of Pathology
and Parasitology, Sylhet Agricultural University, Sylhet,
Bangladesh; 4Department of Zoology, Khilgaon Model
College, National University, Dhaka, Bangladesh; 5Laboratory
of Pathology, College of Veterinary Medicine, Chonbuk National
University, Jeonju, South Korea.
*Corresponding author: bskims@jbnu.ac.kr (B. Kim)
Abstract
This study was conducted to isolate and identify organisms responsible for
sub-clinical mastitis (SCM) in goats and sheep for the determination of point
prevalence of SCM and antibiotic sensitivity of the identified organisms. For
this purpose 50 each of lactating sheep and goats were examined with the
commercially available Leucocytest® SCM detection kit. It was found
that 4 and 36% sheep and goats suffered from SCM, respective. The prevalence of
clinical mastitis (CM) was 4 and 6% in sheep and goats, respectively. Milk
samples were collected individually from sheep and goats with SCM and were
cultured in different media including nutrient agar, blood agar and eosin
methylene blue agar. The bacteria were further characterized by biochemical
tests. In both goats and sheep,
the organisms responsible for SCM were Staphylococcus aureus (S. aureus) and
Escherichia coli (E. coli).
The organisms were found most sensitive to gentamicin (Gn). After Gn
treatment to goats and sheep with SCM, total bacterial counts decreased and milk
production significantly increased compared to levels prior to treatment. It is
suggested from the study that early detection of SCM and treatment with proper
antibiotics can control SCM in goats and sheep.