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Serum Ovarian Steriod Hormones and Some Minerals Concentration in Pregnant Nili-Ravi Buffaloes with or without Pre-Partum Vaginal Prolapse
M. S. Akhtar*, L. A. Lodhi1, I. Ahmad1, Z. I. Qureshi1 and G. Muhammad2
Department of Clinical Sciences, Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan; 1Department of Theriogenology; 2Department of Clinical Medicine and Surgery, University of Agriculture, Faisalabad, Pakistan *Corresponding Author:


The present study was designed with the objective to determine the hormones (estradiol, progesterone) and minerals (calcium, phosphorus, magnesium, sodium and potassium) in serum of buffaloes suffering from pre-partum vaginal prolapse. A total of 200 buffaloes were included in this study, half of these were suffering with pre-partum vaginal prolapse (affected group) between 7th to 10th months of gestation while the remaining were normal pregnant buffaloes (healthy group). Blood samples were collected to procure serum from each animal. Serum hormones and minerals were determined by ELISA technique and direct colorimetric method, respectively. The mean serum estradiol and magnesium concentrations were significantly higher (P<0.01) whereas progesterone, calcium and phosphorus concentrations were significantly lower (P<0.01) in buffaloes suffering with pre-partum vaginal prolapse as compared to healthy pregnant buffaloes. It was concluded that the serum hormones (estradiol and progesterone) and minerals (magnesium, calcium and phosphorus) concentrations differ between the vaginal prolapse affected and healthy buffaloes.

Key words: Buffalo, Calcium, Estrogen, Phosphorus, Progesterone, Vaginal prolapse


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)