College of Fisheries; 1College of
Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070,
China; 2Department of Public Health Sciences,
University
of Hawaii at Manoa, USA
*Corresponding author:xueqinliu@mail.hzau.edu.cn
Abstract
Spring viremia of carp (SVC), caused by spring
viremia of carp virus (SVCV), is an important disease that causes significant
losses in carp fisheries in many countries. The glycoprotein of spring viremia
of carp virus (SVCV/G) is the major viral antigen and plays an important role in
viral infection. In this study, SVCV/G gene was cloned and inserted into the
genome of baculovirus under the control of p10 promoter. SVCV/G was successfully
expressed in insect cells. It was found SVCV/G can mediate insect cell fusion
and SVCV/G that is similar to the glycoprotein of vesicular stomatitis virus
(VSV/G), was detected on the infected insect cell surface by indirect
immunofluorescence assay. The size of SVCV/G expressed in insect cells was
demonstrated to be approximately 66KDa by Western blot. Comparative analysis of
the cell fusions mediated by SVCV/G and VSV/G showed that SVCV/G can cause more
severe insect cell fusions. From the data obtained from this study, we speculate
that SVCV/G may be incorporated into the recombinant baculovirus envelope, and
the recombinant baculovirus harboring SVCV/G on its surface may potentially be
used as an effective vaccine against SVCV.