Effect of Short-Term Administration of Glucagon on Gene Expression
of the Insulin Receptor in Primary Cultured Calf Hepatocytes
Z. G. Zhang§, X. B. Li§1, G. W. Liu§1,
Y. Y. Chen, M. L. Yu, J. G. Wang1, H. B. Wang, L. Liu1,
Y. F. Li, L. Gao, Zhe Wang1*,
L. Liu and X. L. Zhu1
College of Veterinary Medicine, Northeast Agricultural University,
Harbin, 150030, China; 1College of Animal Science and
Veterinary Medicine, Jilin University, Changchun, 130062, China
Contributed equally to this study. *Corresponding author:
wangzhe500518@sohu.com
Abstract
This study investigated whether increased glucagon levels, caused by the
short-term administration of glucagon, lead to an increase in gene expression of
the insulin receptor (InsR) in calf hepatocytes cultured
in vitro.
After 72 hrs of culturing, glucagon
was added to calf hepatocytes at a five
different concentrations of 0, 1, 10, 100 and 1000 nM.
InsR
mRNA expression was determined by
internally controlled reverse transcriptase polymerase chain reaction.
No changes in
InsR mRNA expression (InsR/β-actin gray scale) were detected in hepatocytes
treated with glucagon compared with the control group and there were no
significant differences between the different concentrations. In conclusion,
short-term administration of glucagon did not directly influence the gene
expression of InsR in primary cultured calf hepatocytes.