Occurrence of Co-Infection of
Helicobacter pullorum and
Campylobacter spp. in
Broiler and Village (Indigenous) Chickens
Soe Soe Wai, A. A. Saleha*, Z. Zunita, L. Hassan and A. Jalila
Faculty of Veterinary Medicine, Universiti Putra
Malaysia, 43400 UPM, Serdang,
Selangor, Malaysia
*Corresponding author: saleha@vet.upm.edu.my
Abstract
The reports on prevalence of Helicobacter
pullorum in broiler chickens are rather limited and lacking in village
chickens. This study aimed to determine the occurrence of
H. pullorum in broiler and village
chickens in Selangor,
Malaysia and to
report the detection of co-infection of
H. pullorum and Campylobacter
spp. in these chickens. Village (indigenous) chickens were sampled in five
markets and broiler chickens from six farms in different localities. Cecal
contents were aseptically obtained from the chickens and subjected to three
cultural methods. The isolates were identified by biochemical tests and
confirmed using a species-specific PCR assay.
Helicobacter pullorum were isolated
from 25% village chickens and 24.6% broiler chickens, with an overall occurrence
of 24.7%. Eleven (50%) of these positive chickens (nine in broiler and two in
village chickens) showed co-infection with
Campylobacter spp.