Assessment of Buffalo Bull Semen Quality Based on Sperm
Motility Parameters, Motion Characteristics and
In Vitro Fertilization Rate
A. Sohail, S. M. H. Andrabi1, M. Anwar1,
L. Ali and A. Mehmood1*
Department of Animal Genomics and Biotechnology,
PARC Institute of Advance Studies in Agriculture, Islamabad; 1Animal
Sciences institute, National Agricultural Research Centre, Islamabad
4500, Pakistan
*Corresponding Author: mehmood_apk@yahoo.com
Abstract
The objective of the present study was to
determine the prognostic value of sperm motility parameters and motion
characteristics to predict in vitro
fertilization (IVF) rate in the buffalo. Sperm motility parameters (%; motile,
progressive, rapid and medium)and motion
characteristics (path velocity = VAP, µm/sec; progressive velocity = VSL,
µm/sec; curvilinear velocity = VCL, µm/sec; amplitude of lateral head = ALH, µm
and beat cross frequency = BCF, Hz) of 5 buffalo bulls (Tag no. 2, 4, 6, 11 and
12) were determined by Cell Motion Analyzer (CASA) at
post dilution
(PD), pre freezing (PF) and post thaw (PT) stages. IVF ability of
individual bull (n = 5) was determined by cleavage rate (CR, %). Multiple
regression analysis was used to determine the prognostic values of sperm
motility parameters and motion characteristics for CR as a dependent variable.
At PT, Bull 2 showed the highest (P<0.05) progressive and rapid motility, and
VAP, VSL and VCL values as compared to other bulls.
Data of CR (%) were similar among the five buffalo bulls. However, the
prognostic value to predict the cleavage rate by the equation
of motility and motion characteristics accounted for 81% (P=0.01). In
conclusion, although at PT, Bull 2 showed the highest sperm progressive
and rapid motility parameters, and motion characteristics (VAP, VSL and VCL) as
compared to other bulls, but the CR was
similar. The prognostic value by the equation
of motility and motion characteristics accounted significantly to predict the
CR.
Key words: Buffalo bulls,
CASA,
Cleavage rate,
Prognostic value