Leptospirosis is an important zoonotic problem occurring globally. To study the serosurveillance of canine leptospirosis, 429 dogs (265 males; 164 females) blood samples were collected around Lahore city and its peri-urban areas and processed by using canine Leptospira IgG ELISA kit. Of 429 samples, 36.1% were positive serologically for canine leptospirosis. Among 265 male and 164 female dogs, 38.5 and 32.3% male and female dogs were found positive, respectively. Canine leptospirosis was more (38.4%) in adults as compared to pups (27.9%). While season wise serosurveillance was 21.2, 35.8, 40.3 and 49.3% in winter, spring, summer and fall, respectively. It is concluded that highest prevalence of canine leptospirosis is recorded in summer and fall months.

Key words: Canine leptospirosis, Prevalence, Serosurveillance, Temperature