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Evaluation of BSK-H Complete Medium Supplemented with Rabbit Serum and Sodium Bicarbonate for the Growth of Borrelia anserina
Bilal Aslam, Iftikhar Hussain*, Muhammad Shahid Mahmood and Ahrar Khan
Institute of Microbiology, 1Department of Pathology, University of Agriculture, Faisalabad, Pakistan *Corresponding author:


The present study was conducted to compare the effect of 3 formulations of BSK (Barbour-Stoenner-Kelly) medium on the growth of Borrelia (B.) anserina, the causative agent of avian borreliosis. Three different formulations of BSK medium (BSK-H, BSK-II and BSK-H complete medium) were prepared. For the isolation of B. anserina, Argas ticks were inoculated in all the three (BSK-H, BSK-II and BSK-H complete) formulations of BSK medium. All the samples were also observed for the impact of BSK medium on the growth (Generation time, Growth per hour, Specific growth rate) of B. anserina. Phase contrast microscopy was performed for the observation of viable B. anserina cells, and additional confirmation of all the isolates was done by performing indirect immunoflourescent assay (IFA) and polymerase chain reaction (PCR). BSK-H complete medium supplemented with 6% rabbit serum and sodium bicarbonate was found best when compared to two other formulations of BSK medium with respect to the isolation, generation time and growth rate of Borrelia spirochaetes.

Key words: Borrelia anserina, BSK medium, Fowl spirochaetosis


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)