Evaluation of BSK-H Complete Medium Supplemented
with Rabbit Serum and Sodium Bicarbonate for the Growth of
Borrelia anserina
Bilal Aslam, Iftikhar Hussain*, Muhammad Shahid
Mahmood and Ahrar Khan
Institute of Microbiology,
1Department of Pathology,
University
of Agriculture, Faisalabad, Pakistan
*Corresponding author: driftikharuaf@gmail.com
Abstract
The present study was conducted to compare the
effect of 3 formulations of BSK (Barbour-Stoenner-Kelly) medium on the growth of
Borrelia (B.) anserina, the causative agent of avian borreliosis. Three
different formulations of BSK medium (BSK-H, BSK-II and BSK-H complete medium)
were prepared. For the isolation of B.
anserina, Argas ticks were inoculated in all the three (BSK-H, BSK-II and
BSK-H complete) formulations of BSK medium. All the samples were also observed
for the impact of BSK medium on the growth (Generation time, Growth per hour,
Specific growth rate) of B. anserina.
Phase contrast microscopy was performed for the observation of viable
B. anserina cells, and additional
confirmation of all the isolates was done by performing indirect
immunoflourescent assay (IFA) and polymerase chain reaction (PCR). BSK-H
complete medium supplemented with 6% rabbit serum and sodium bicarbonate was
found best when compared to two other formulations of BSK medium with respect to
the isolation, generation time and growth rate of Borreliaspirochaetes.