The present study was conducted to evaluate the possible toxic effects of xylanase enzyme on various hematological and some serum biochemical parameters in broiler chicks. For this purpose seventy five broiler birds were randomly divided into five equal groups (A-E) each having 15 birds. Xylanase was given orally @ 0, 250, 500, 750 and 1000 IU/bird/day for 28 days. The blood samples with and without anticoagulant (EDTA; 1mg/ml) were collected from all the birds for hematological and serum biochemical analysis. Non-significant results were recorded for erythrocyte, leukocyte and platelets count as well as total protein, hemoglobin concentration and hematocrit values. Similarly, the liver, kidneys and cardiac enzymes, serum minerals and lipid profiles were not significantly different in all treated groups as compared to control. The results of present study revealed that xylanase treatments had non-significant effects on kidneys, heart and blood forming tissues exhibiting its safety for food applications.

Key words: Broiler, Hematology, Serum, Xylanase