Interleukin 10
Suppresses the Function of Mouse Bone Marrow-Derived Dendritic Cells
Infected with Classical Swine Fever Virus C-Strain
Fu-Ying Zheng, Guo-Zhen Lin* and Zhi-Zhong Jing
State Key Laboratory of Veterinary Etiological
Biology, Lanzhou Veterinary Research Institute, Chinese Academy of
Agricultural Sciences, No. 1 Xujiaping, Yanchangbao, Lanzhou, Gansu
Province 730046, China *Corresponding author’s e-mail: lgzh18@163.com
Abstract
Interleukin (IL)-10 inhibits the functions of
antigen-presenting cells (APCs), including dendritic cells (DCs), however, the
precise mechanism of action of IL-10 has not been fully elucidated. In this
work, the effects of IL-10 on classical swine fever virus (CSFV)
C-strain-infected mouse bone marrow-derived immature DCs (BM-imDCs) were
studied. Additional IL-10 suppressed the maturation of the infected BM-imDCs by
down-regulating the expression levels of the surface molecules CD80, CD86 and major histocompatibility complex (MHC) classII,
while the autocrine IL-10 had no significant effect on the maturation status of
the cells. Both additional and autocrine IL-10 markedly inhibited the secretion
production of IL-12P40 derived from the BM-imDCs infected with the C-strain, and
reduced the capacity of DCs to promote
allogeneic naive T cell proliferation. These results showed that IL-10 may play
an important role in the DCs-dependent immune response induced by CSFV C-strain.