A research study was conducted to investigate the effect of high dose of recombinant bovine somatotropin (rbST) on the serum biochemical, liver enzymes and hormones of Nili Ravi buffaloes. Clinically healthy lactating buffaloes (n=16) were selected at the livestock farm, university of agriculture, Faisalabad, Pakistan. They were divided into two groups in an equal number. One served as experimental group (n=8) and the other as a control (n=8). Dose of 500 mg rbST/16 days/animals was injected subcutaneously to the experimental group subcutaneously, twice during the experimental period of 32 days after an interval of 16 days. Serum biochemical parameters (glucose, cholesterol, total proteins, urea and globulin), serum hormones (T₃, T₄, cortisol) and liver enzymes (AST, ALT) were estimated. Glucose was significantly high while total proteins, globulin and urea were significantly low in the treated group. Significantly high ALT and low T₄ level were recorded in the bST treated group. It was concluded from this study that the studied parameters did alter in buffaloes with 500 mg rbST injection.

Key words: Buffaloes, Liver enzymes, rbST, Serum biochemistry, Serum hormones