Genetic polymorphisms of Mc4R and IGF2 gene association with feed conversion efficiency traits in beef cattle
Xin-hua Du§, Cui Chen§,
Zheng-rong Yuan, Li-min Zhang, Xiao-jie Chen, Yan-hui Wang, Xue Gao,
Lu-pei Zhang, Hui-jiang Gao, Jun-ya Li and Shang-zhong Xu*
Institute of Animal Science, Chinese Academy of
Agricultural Sciences,
Beijing, People’s Republic of China *Corresponding author:simmenta@vip.sina.com
Abstract
Melanocortin-4 receptor (MC4R) gene is part of the central melanocortin pathway located in
the hypothalamus, an area of the brain in which appetite is regulated.
Insulin-like growth factor 2 (IGF2)
gene plays a role in muscle growth, myoblast proliferation and differentiation.
Thus, they are candidate genes for feed conversion efficiency (FCE). The study
was to investigate the effects of variants in cattle
MC4R and IGF2 gene on FCE
traits including residual feed intake (RFI), feed conversion ratio (FCR) and
average daily gain (ADG). We screened single nucleotide polymorphisms (SNPs) of
the two genes in 118 Simmental bulls by DNA-pool sequencing and genotyped by
matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
(MALDI-TOF-MS) analysis. C1069G locus
of MC4R and four SNPs (C2209T,
G18587C, A22950T and
G26920T) of
IGF2 were identified in the population. The χ2 test showed that only
MC4R-C1069G,IGF2-C2209T and
IGF2-G18587C loci fitted with Hardy-Weinberg equilibrium (P>0.05).
General linear model (GLM) was used to analyze differences between genotypes.
The results showed that
only IGF2-G18587C locus has a
significant effect on ADG (P<0.05), but has no significant effect on RFI or FCR (P>0.05). CC and GG
genotypes were the dominant genotypes; individual with CC or GG genotype had a
larger ADG than GC (P<0.05).