The effects of resveratrol on arsenic trioxide-induced oxidative stress and arsenic efflux in spleen were determined in Wistar rats exposed to arsenic trioxide (As₂O₃). Wistar rats were segregated into four groups: control (0.9% normal saline), As₂O₃-treated (2 mg/kg for 7 days, i.p.), resveratrol+As₂O₃-treated, resveratrol-treated (5 mg/kg for 7 days, i.p.). Spleen was analyzed for lipid peroxidation, antioxidant enzyme activities, glutathione redox system and arsenic accumulation. Arsenic treatment showed significant increases in the malondialdehyde, reactive oxygen species and arsenic accumulation in spleen. Furthermore, arsenic treatment decreased significantly activity of superoxide dismutase and total glutathione/ oxidized glutathione ratio. However, treatment with resveratrol to arsenic-treated rats reduced oxidative stress and improved arsenic efflux. These findings may provide the potential strategy for arsenic-induced spleen toxicity and As₂O₃ as a single agent causing toxic effects.

Key words: Arsenic efflux, Arsenic trioxide, Oxidative stress, Resveratrol