Chatchai Sarachai1,
Jiroj Sasipreeyajan2 and Niwat Chansiripornchai2,3*
1Department
of Food Animal Clinic, Faculty of Veterinary Medicine, Chiang Mai
University, Chiang Mai 50100, Thailand; 2Avian Health
Research Unit, Department of Veterinary Medicine, Faculty of
Veterinary Science, Chulalongkorn University, Bangkok 10330,
Thailand; 3World
Organisation for Animal Health (OIE), 12 Rue de Prony, 75017 Paris,
France.
*Corresponding author: cniwat@chula.ac.th
Abstract
The purpose of this study was to prepare and
characterize virosome containing envelope proteins of the avian influenza (H5N1)
virus. The virosome was prepared by the solubilization of virus with
octaethyleneglycol mono (n-dodecyl) ether (C12E8) followed
by detergent removal with SM2 Bio-Beads. Biochemical analysis by SDS-PAGE and
western blotting, indicated that avian influenza H5N1 virosome had similar
characteristics to the parent virus and contained both the hemagglutinin (HA,
60-75 kDa) and neuraminidase (NA, 220 kDa) protein, with preserved biological
activity, such as hemagglutination activity. The virosome structure was analyzed
by negative stained transmission electron microscope (TEM) demonstrated that the
spherical shapes of vesicles with surface glycoprotein spikes were harbored. In
conclusion, the biophysical properties of the virosome were similar to the
parent virus, and the use of octaethyleneglycol mono (n-dodecyl) ether to
solubilize viral membrane, followed by removal of detergent using polymer beads
adsorption (Bio-Beads SM2) was the preferable method for obtaining avian
influenza virosome. The outcome of this study might be useful for further
development veterinary virus vaccines.