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Variability of Point Mutation Rates in Campylobacter coli: Preliminary Data and Observations
Anna Caroli1, Elena Circella1, Carlo Pazzani2, Antonio Camarda1*, Marilisa Legretto1 and Nicola Pugliese1
1Department of Veterinary Medicine, University of Bari “Aldo Moro”, SP per Casamassima, km 3, 70010 Valenzano (Ba), Italy; 2Department of Biology, University of Bari “Aldo Moro”, Via Orabona, 4, 70125 Bari, Italy
*Corresponding author:


In this study we evaluated the in vitro variability of flaA in Campylobacter coli strains (Co19 and Co29) isolated from distinct poultry farms. Strains were characterized by flaA PCR-RFLP and subcultured in absence or presence of Salmonella enterica (S. Gallinarum) as a host competitor in poultry. Only transition mutations were detected and no flaA-flaB recombination events were observed. The highest ratio of flaA mutations was detected in the presence of competitor (1x10-6 and 4.8x10-6 for Co19 and Co29, respectively). While, transitions in absence of competitor were observed only in the strain Co29 (5.8x10-7). Data obtained in this investigation represent a preliminary study aimed to evaluate the possible linkage between specific haplotypes and their proneness to acquire point mutations which in turn might help strains to gain a better fitness for survival within and outside hosts.

Key words: Campylobacter coli, Competitive stress,  flaA, Variability


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)