Involvement of Caspase-Dependent
and Independent Pathway in Apoptotic Action of Extrinsic β-Hydroxybutyrate
in Liver Tissue of Mice
Teng Wei§, Mingfang Bi§,
Fangning Liu§, Tingting Lv§, Wulin Tian§
and Guanghong Xie*
College of Veterinary Medicine, Jilin University, Changchun
130062, China *Corresponding author:xiegh@jlu.edu.cn
Abstract
β-hydroxybutyricacid (β-HB) is generated by
degradation of fatty acids in the liver. β-HB level in serum increases
significantly in a variety of metabolic disorder diseases, such as
hyperketonemia and diabetic ketoacidosis, in which hepatocyte apoptosis have an important role. To investigate the
effect of exogenous β-HB on the liver, mice were injected intraperitoneally with
β-HB (7.5 mmol/kg) one time, three times and five times at 24 h intervals,
respectively. Compared to the distilled water injection group, β-HB level in
liver tissue increased significantly, while the triglycerides, high density
lipoprotein cholesferol (HDL), low density lipoprotein cholestorol (LDL),
alkaline phosphatase (ALP) in serum have not changed in the three β-HB injection
groups. β-HB promoted the activation of caspase-12, caspase-9, caspase-3 and
EndoG, up-regulated Bax and down-regulated of Bcl-2.