Hsp-90 Inhibitor Geldanamycin Attenuates Liver
Oxidative Stress and Toxicity in Thiram-Induced Tibial
Dyschondroplasia
Muhammad Shahzad1, 2, Jingying Liu1,
Jianfeng Gao1, Zhi Wang1, Ding Zhang1,
Fazul Nabi1 and Jiakui Li1*
1College
of Veterinary Medicine, Huazhong Agricultural University, Wuhan
430070, PR China; 2University College of Veterinary &
Animal Sciences, The Islamia University of Bahawalpur 63100,
Pakistan *Corresponding author:
lijk210@sina.com
Abstract
An experiment was conducted to study the effects
of hsp90 inhibitor geldanamycin (17-DMAG) on liver functioning and its
antioxidant ability in thiram-induced tibial dyschondroplasia. One hundred and
twenty commercial chicken broilers were allocated into three groups: 1) control,
2) thiram-induced and 3) 17-DMAG treated. Serum samples were collected on day 11
and 14 post-hatch to determine the liver ALT, AST and ALP activity. The liver
samples were collected at the end of trial to determine the activity of SOD
(superoxide dismutase), GSH-Px (glutathione peroxidase) and MDA (malondialdehyde)
contents. The results depicted that thiram increased the level of serum ALT, AST
and liver MDA contents while decreased the serum ALP and liver antioxidant
enzymes (SOD, GSH-Px); however, by administering 17-DMAG, these values were
observed close to normal range as compared to control group. In conclusion, the
oxidative imbalance and damage to liver caused by thiram can be restored by
using 17-DMAG.