Adipogenic Gene Expression and Regulation Pattern
in Adipose Tissues Located on Mammary, Inguinal and Renal Fat Pad of
Korean Black Goat (Capra
hircus)
S Subi1, NK Singh1*, AMMT Reza1,
S Shiwani1, JD Lohakare1, DK Jeong2
and SJ Lee1
1Department
of Animal Biotechnology, College of Animal life sciences,Kangwon National University; 2Department of Animal
Biotechnology, College of Applied life Sciences, Jeju National
University, Republic of Korea;
*Corresponding author:singh@kangwon.ac.kr
Abstract
Development of new fat cells appears to be a
permanent event throughout the life process of both humans and animals. Though,
this process appears to be similar all over the body, but whether their
anatomical location keeps them distinct in their genetic makeup is quite
uncertain. Therefore, to investigate the difference in the adipogenic gene
expression and their regulation pattern at the molecular level, we collected
adipose tissues surgically from prepubertal Korean black goat reared in stall
fed system and with high fat rich diet. Perirenal fat, inguinal fat and mammary
fat pad adipose tissues were collected and cyropreserved in LN2 for mRNA
isolation subsequently. Reverse transcriptase PCR revealed significant (P<0.05)
down-regulation of adipo Q in mammary fat pad and inguinal fat, but no change
was observed in renal fat in the high fat rich diet fed group as compared to the
control group. Significant down-regulation in LPL and resistin gene was noticed
in parallel in all the tissues in the treatment group. However, PPAR-γ was
observed to be significantly down-regulated only in perirenal fat and whereas
CEBP-α was down-regulated in both inguinal and perirenal fat in the high fat
rich diet fed goats as compared to the control. Down-regulation of specific
adipogenic gene clearly shows that optimization of high fat rich diet in dairy
goat management is highly essential. This
further suggests that adipose tissue participates more directly than previously
thought in metabolic activities and energy balance.
Key words:
Adipose tissues,
Inguinal,
Korean black goat,
Mammary fat pad, Peri-renal