Histomorphometric and Hematological Profile of Grass Carp
(Ctenopharyngodon
idella) during Acute Endosulfan
Toxicity
Zaigham Hasan, Shahzad
Ghayyur, Zahoor Ul Hassan1* and Shahid Rafique2
Department of Zoology,
University of Peshawar-25120, Pakistan; 1Department of
Animal Health, The University of Agriculture Peshawar; 2Animal
Sciences Division, Pakistan Agricultural Research Council,
Islamabad, Pakistan;
*Corresponding author: zahoor82@gmail.com
Abstract
This study was
designed to investigate the acute toxicities of endosulfan on the hematopoietic,
gastro-intestinal, respiratory systems of grass carp (Ctenopharyngodon idella). A total of 35 grass carp, 5-6 cm long were
divided into five groups (E0, E1, E2, E3 and E4) having seven fish in each. The
fish in group E0 were kept as control in plain water, while those in group E1,
E2, E3 and E4 were exposed to 0.75, 1.00, 1.50 and 2.00 ppb of endosulfan in
water, respectively for 96 hrs. Water and test chemical solution were renewed
daily throughout the experiment. At the end of experimental period blood samples
from fish in each group were collected. The morphometric parameters including
total weight, length, standard length and organ weights showed non-significant
difference. Samples from gills, intestine and liver were collected for the
histopathological examination. A significant dose dependent decrease was noted
in the leukocyte and erythrocyte counts, while the platelets were higher in the
intoxicated fish. The values of Hb and PCV were also significantly lower in the
high dosed group. There was fusion and disruption of gills along with severe
congestion in the high dosed fish groups. The intestine showed atrophy of villi
and increased goblet cells count. The hepatocytes showed degenerative changes
including vacuolation, pyknosis etc. It was concluded that use of endosulfan in
the water is extremely toxic for the fish health so a strong legislation must be
implemented in the developing world as in the analogy of European Union.