Anthelmintic Activity of Ellagic Acid, a Major
Constituent of Alternanthera
sessilis Against
Haemonchus contortus
Himangsu Mondal1,
Hemayet Hossain2,
Khalijah Awang3,4,
Sanjib Saha1,
Sheikh Mamun-Ur-Rashid1, Md Khirul Islam1, Md. Sohanoor Rahman1,
Ismet Ara Jahan2, Mohammad Mahfuzur Rahman2
and Jamil A. Shilpi1,4*
1Pharmacy Discipline, Life
Science School, Khulna University, Khulna 9208, Bangladesh; 2BCSIR Laboratories& IFST, Bangladesh Council of
Scientific and Industrial Research, Dhaka 1205, Bangladesh; 3Department
of Chemistry, Faculty of Science, University of Malaya, Kuala Lumpur
50603, Malaysia; 4Centre
for Natural Products and Drug Discovery, University of Malaya, Kuala
Lumpur 50603, Malaysia *Corresponding
author:jamilshilpi@yahoo.com; jamilshilpi@um.edu.my
Abstract
Alternanthera sessilis,also known as ‘sessile joyweed’ or ‘dwarf copperleaf’,
is a popular vegetable and used in traditional medicine in some Asian countries
including Bangladesh for the treatment of various ailments. Anthelmintic
activity of the ethanol extract of A. sessilis (ASE) and one of its major constituents ellagic acid
(EA) was tested against
cattle nematode Haemonchus contortus by adult motility test and
egg hatch assay. In adult motility test, both ASE (1.56-50
mg/ml) and EA
(0.09-3 mg/ml)
showed a concentration dependent inhibitory effect on H. contortus.
All test worms died 6 h post-exposure of 12.5 mg/ml of ASE treatment and
6 h post-exposure of 1.5 mg/ml of EA treatment. For the concentration of 1.5
mg/ml of the reference drug albendazole, all test worms died 2 h post-exposure
of the treatment. In egg hatch assay, both ASE (0.0125-25 mg/ml) and EA
(0.0125-25 µg/ml) showed a concentration dependent inhibition of the larval
production from H. controtus eggs with
the LC50 value of 150.00 and 3.097 µg/ml, respectively. The LC50
for albendazole (0.0125-25 µg/ml) was 0.163 µg/ml. In the HPLC analysis, EA,
rutin, (+) catechin and quercetin (3007.26,
490.74, 117.72 and 13.85 mg/100 g extract, respectively) were detected. Phytochemical group test of ASE indicated the presence
of reducing sugars, steroids, terpenoids, saponins, tannins and flavonoids.
Thus, high level of EA in ASE, along with other
phytochemical constituents might be responsible for the observed activity of the
extract.