Seasonal Variations in Histomorphology of Testes
and Bursa, Immune Parameters and Serum Testosterone Concentration in
Male Guinea Fowl (Numida
meleagris)
Malik Zohaib Ali1*, Anas Sarwar
Qureshi1, Sarmad Rehan1, Syed Zeeshan Akbar2
andAsad Manzoor3
1Department
of Anatomy; 3Department of Clinical Medicine and Surgery,
University of Agriculture, Faisalabad 38040, Pakistan; 2Department of Veterinary Biomedical Sciences, Pir Mehr Ali Shah (PMAS)
Arid Agriculture University Rawalpindi, Pakistan;
*Corresponding author:
drmalikzohaibali@gmail.com
Abstract
The objective of this study was to investigate
the effect of photoperiod on histomorphological changes in testes and bursa,
immune function parameters like total leukocyte count and lymphocyte count and
serum testosterone level of guinea fowl (Numida
meleagris) during different breeding seasons. Bursa and testes were
collected from 10 mature male guinea fowls aged 6-8 months during full breeding
(summer), low breeding (autumn) and non-breeding (winter) seasons (n=10).
Tissues were stained with H&E and Image J® software was used for
histometric analysis. Statistical analysis revealed that breeding seasons
affected significantly (P<0.01) all morphological parameters of testes and
bursa. The parameters of testes were found significantly higher during full
breeding season which decreased significantly during non-breeding season through
low breeding season. A similar trend was shown by diameter of testicular
seminiferous tubules. However, the parameters of bursa showed highly significant
(P<0.01) value during non-breeding season but this value declined
non-significantly (P>0.05) during low breeding and full breeding seasons. Immune
parameters exhibited a significantly higher value during non-breeding season
with a significantly declining trend during low breeding and full breeding
seasons. Serum testosterone levels were also significantly (P<0.01) different
during all breeding seasons. Testosterone showed a negative correlation (r
=-0.946) with immune function parameters. It is conceivable that increased
steroid hormone synthesis enhances testicular activity during full breeding
season but depresses the immune function. This trend however, reversed in
non-breeding season, which assists the survival in the hard climatic conditions.