Leptin Gene Polymorphism in Lohi, Kajli and Spili
Breeds of Sheep
Zafar Iqbal Qureshi1, A Hossain Farid2,
Masroor Ellahi Babar3 and Tanveer Hussain3,4
1Department
of Theriogenology, Faculty of Veterinary Science, University of
Agriculture Faisalabad Pakistan; 2Department of Plant and
Animal Sciences, Faculty of Agriculture, Dalhousie University,
Truro, Canada; 3Department of Molecular Biology, 4Virtual
University of Pakistan, Lahore *Corresponding author: zafaruaf2013@gmail.com
Abstract
Pakistan is blessed with rich sheep genetic
resources being a source of milk, meat and wool across the country. There has
been a very limited work on sheep genetic exploration. The Leptin a 16-kDa
protein has been involved milk yield, energy balance, feed intake regulation,
fertility and immune functions in animals. This gene is getting importance for
animal researches to find useful biomarker for identification of high performing
animals with better adaptability and productivity. In the present study, the
polymorphism of the leptin gene in Lohi, Kajli and Sipli famous sheep breeds of
Pakistan was analyzed by PCR-RFLP technique using six restriction enzymes (BbvI,
SfcI, AccI, Sty1, BsmF1, Ban II) that showed monomorphic pattern in intron 2
(position A2262T, C2256G, -2730C) and exon 2 (A3201G) while polymorphic trend
was observed in intron 2 (at position C1467T and A3050-). BbvI was found monomorphic Lohi and Sipli sheep while polymorphic in Kajli
sheep at position C1467T.
Interestingly the Sty1 enzymes was proved polymorphic (A/-) at position 3050 in Lohi, Kajli and Sipli sheep breeds of Pakistan. The
SfcI, AccI, BsmF1 and Ban II
enzymes showed monomorphic results in all breeds. The different production traits can be associated with
leptin genotypes in future and we recommend that polymorphisms in Leptin gene
may be used to develop useful markers for selection of better animals under
Maskers Assisted Selection program.