Aim of the present study was to determine the occurrence of Campylobacter in cattle and their farms environment at two different incubation temperatures. A total of 248 samples including cattle (180), water (27), feed (7), cattle house floor (9), water trough (9) and flies (16) were collected from dairy and beef farms in Malaysia. All samples were cultured on Campylobacter blood free selective agar base (mCCDA- modified Charcoal Cefoperazone Deoxycholate Agar) and isolates were identified using multiplex PCR. Prevalence of Campylobacter spp. was 26.1% in cattle. Campylobacter spp. were isolated from 37, 33.3, 25, 14.3 and 11.1% of water, floor of the cattle houses, flies, feed and water trough samples, respectively. The overall isolation rates of Campylobacter was higher at 42ºC (25.4%) as compared to 37ºC (20.6%); however, the difference was not significant (P>0.05). It is concluded that Campylobacter are quite prevalent in cattle and farm environment which could be a potential source of human infection and environmental contamination.

Key words: Beef, Campylobacter, Dairy, Farm environment, Incubation temperatures, Occurrence