1Department
of Anatomy, Faculty of Veterinary Science, Chulalongkorn University,
Bangkok, 10330 Thailand; 2Department of Surgery and
Theriogenology, Faculty of Veterinary Medicine, Khon Kaen
University, Khon Kaen, 42000 Thailand; 3Department of
Clinical Sciences and Public Health, Faculty of Veterinary Science,
Mahidol University, Salaya, Nakhon Pathom, 73170 Thailand *Corresponding author: sukanya.man@mahidol.edu
Abstract
The open and closure of cervical canal is an
important function in both reproductive physiological and pathological
processes. The cervical dilation during the estrous cycle is involved the
presence of oxytocin receptor (OTR) in most species. The regulation of cervical
dilation during the estrous cycle in bitch has not been exactly reported. This
study aimed to investigate the expression of OTR in bitch cervical tissue during
various stages of the estrous cycle using immunohistochemical technique.
Thirty-one bitches were divided into three groups, follicular, luteal, and
inactive phase according to ovarian structures. The results presented as the
mean percentage of the positive staining area showed that the OTR expression was
mostly found in the cytoplasm of all cervical compartments. The highest
percentage of OTR positive staining area was observed in the follicular phase
group (P<0.05), whereas the lowest OTR expression was observed in the inactive
phase group (P<0.05). The present study suggested that the OTR expression is
influenced by the phase of the estrous cycle and that cervical cells are the
target sites of oxytocin during the estrous cycle. Therefore it may also
conclude that the action of oxytocin may play a role in the mechanism of the
cervical dilation in cyclic bitches through the expression of its receptor.