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Comparison of Cell Markers Expression in Canine and Human Spontaneous Osteosarcomas and Established Osteosarcoma Cell Lines
Dominik Poradowski1*, Rafał Ciaputa2, Bożena Obmińska-Mrukowicz1, Marcin Nowak2, Elżbieta Górzyńska3and Izabela Janus2
1Department of Biochemistry, Pharmacology and Toxicology, Division of Pharmacology and Toxicology; 2Department of Pathology, Division of Pathomorphology and Veterinary Forensic Science, Wrocław University of Environmental and Life Sciences, ul. C.K. Norwida 31, 50-375 Wrocław, Poland; 3Histopathological Laboratory Hist-Med. s.c.N.Z. M. Kosiński. P.Prajs, 51-124 Wrocław, Poland
*Corresponding author:


The aim of the study was to demonstrate and analyze the expression of the following proteins, β-catenin, E-cadherin, vimentin, Ki-67, mini-chromosome maintenance 3 (MCM3), mini-chromosome maintenance 7 (MCM7), osteocalcin, cyclooxygenase-2 (COX-2), and microsomal PGE2 synthase-1 (mPGES-1) in the spontaneous osteosarcomas isolated in dogs and in humans, and to compare the results with these obtained in the established canine (D-17) and human (U-2 OS) cell lines. Immunohistochemical tests were conducted on 4 µm -thick paraffin sections and cells from established canine and human osteosarcoma cell lines. The sections and cells from cell lines were overlayed with primary antibodies. The immunocytochemical reaction was induced by 3,3 diaminobenzidinetetrahy-drochloride (DAB) solution. In a number of cases, the expression of the investigated cell markers was of comparable intensity in the cell lines and spontaneous osteosarcomas, except β-catenin and mPGES-1 in dogs. There were also no significant differences in the expression of the investigated cell markers between the group of humans and dogs. It is also worth noting that the obtained results open a perspective for developing an appropriate animal model for the study of oncogenesis in humans.

Key words: Dog, Expression of proteins, Man, Osteosarcoma cell lines


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)