An Assessment of the Application of Poloxamer/Sodium
Alginate/CaCl2 Mixture after Abdominal Surgery in Dogs:
Effects on Postoperative Adhesion and Safety
Jina Kim, Sohee Bae, Li Li, Junemoe Jeong, Aeri
Lee, Hyunjoo Lim, Oh-kyeong Kweon and Wan Hee Kim*
Department of Veterinary Clinical Science,
College of Veterinary Medicine, Seoul National University, 1
Gwanak-ro, Gwanak-gu, Seoul 151-742, Korea;
*Corresponding author: whkim@snu.ac.kr
Abstract
Poloxamer/sodium alginate/CaCl2
(PX/SA) mixture (Aniguard®, Genewel, South Korea) is a
temperature-sensitive anti-adhesive agent used to prevent postoperative adhesion
by forming a biocompatible barrier on the surface of a wound. This study
investigated the safety and the ability of PX/SA mixture to prevent
postoperative adhesion in dogs. Twenty dogs were divided into two experimental
models: an intestine model and a uterus-urinary bladder model. Each model was
further divided into two groups. Dogs in the intestine model underwent
laparotomy, with multiple intestinal wall abrasions, while dogs in the
uterus-urinary bladder model underwent urinary bladder wall abrasion. The
control groups were treated with normal saline, and the trial groups were
treated with PX/SA mixture. After 10 days, all dogs underwent a laparoscopic
exploration to evaluate their abdominal status. On postoperative day 21, the
abdominal adhesions were evaluated and scored by a previously validated adhesion
score system. Furthermore, the extent of fibrosis and inflammation was scored
according to a histopathologic examination. The adhesion scores of the trial
groups were significantly reduced in both models compared to those of the
control groups. Furthermore, more fibrosis reactions were found on the adhesion
areas in the control groups in both models. There were no statistically
significant differences in inflammation between the two groups. In addition, no
hepatic and renal toxicity or side effects of PX/SA mixture were observed. In
conclusion, the use of PX/SA mixture effectively reduced the development of
postoperative abdominal adhesions.