Expression Pattern of Stem Cell Markers in Developing Mouse Pancreas
Thanaa A Nugali1,
Siham K Abunasef2,3,
SalehAMKarim4,
Fatma Al-Qudsi4 and Waqas Iqbal*4
1Biology
Department, Umm Al Qura University, Mecca, Kingdom of Saudi Arabia;
2Anatomy and Histology
Departments, Faculty of Medicine, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia; 3Faculty of Medicine, Ain
Shams University, Cairo, Egypt; 4Biology Department, Faculty of Science, King Abdulaziz
University, Jeddah, Kingdom of Saudi Arabia;
*Corresponding author:waqasbiotech@yahoo.com
Abstract
Identification of stem cells in-vivo opens up the
possibility of their expansion in-vitro, exploiting their multipotency in
treating diabetes type 1 and type 2. Little is known about the relationship
between a common pancreatic transcription factor (Pdx-1) and stem cell markers
(CK19, CD29, CD56) in mice during pancreatic organogenesis. In this study the
focused was on the prenatal immunohistochemical expression of stem cell markers
(Pdx-1, CK19, CD29, and CD56) with special reference to their site and degree of
expression during the prenatal pancreatic organogenesis. Material and Methods:
Whole embryos and pancreatic tail of different prenatal groups (days 13, 15, &
18) were stained by H&E and immunohistochemically stained for Pdx-1 and stem
cell markers, CK19, CD29, and CD56. Data was statistically analyzed for the
evaluation of changes in the pattern and the degree of expression of stem cell
markers in the pancreas. Results: Pdx-1 a transcription factor with role in
pancreatic organogenesis was expressed in the duct, acini and islets in all
ages. CK19 was expressed in the duct at day 13 and 15 prenatally, but in islet
at day 18. Acinus and islets were positive for CK19 at all ages. CD29 on the
other hand had positive expression in the duct while acini and islets had it at
day 18 only. The acinar and islet cells were positive for CD56 at day 18 only.
In conclusion, PDX-1 a transcription factor is vital in early pancreatic
organogenesis whereas CK19, CD26 and CD56 are purportedly involved in generation
of β-cells.