Effect of Cadmium on the Concentration of
Ceruloplasmin and its mRNA Expression in Goats under Molybdenum
Stress
Yu Zhuang§, Ping Liu§,
CaiYing Zhang, Shu Ye, GuoLiang Hu* and HuaBin Cao*
Institute of Animal Population Health, College of
Animal Science and Technology, JiangXi Agriculture University, N.O.
1101, Zhimin Avenue, Nanchang Economic and Technological Development
District Nanchang 330045, P.R. China *Corresponding author:hgljx@163.com
(GLH); chbin20020804@163.com (HBC)
Abstract
The present study aim at evaluating the effects
of cadmium on the concentration of ceruloplasmin (CP) and its mRNA expression in
goats under molybdenum stress. CdCl2 and [(NH4)6Mo7O24·4H2O]
were selected as the source of cadmium and molybdenum, respectively. Thirty-six
healthy goats were divided into four groups randomly, including control group
(0mg/kg Mo+0mg/kg Cd), Mo exposure group (30mg/kg Mo),
co-exposure group A (30mg/kg Mo+0.5mg/kg Cd) and co-exposure group B (30
mg/kg Mo+1 mg/kg Cd). Fifty days after exposure, blood and liver samples were
collected. The results showed that all animals in experimental groups had shown
some visible clinical signs of copper deficiency. The body weight and activity
of CP protein in those animals decreased significantly as well. Meanwhile, Mo,
Cd and Cu were significantly increased in the liver in all treatments, and
contents of Mo, Cd and Cu in serum and liver of animals in co-exposure (Mo+Cd)
groups were significantly higher than those in Mo exposure group. In addition,
the accumulated level of cadmium positively correlated with time and dose.
However, the mRNA expression of ceruloplasmin in Mo exposure group increased
significantly (P<0.05), while in co-exposure groups, the level of expression
increased before day 25 and then decreased rapidly. This study suggests that Cd
has a promotional effect on molybdenosis which involves in aggravating the
deposition of heavy metals (Mo, Cd and Cu).