Gastric
Helicobacter-like Organisms in Stray Cats: Identification,
Prevalence, and Pathologic Association
Serpil Dağ1*, Mahmut Sözmen2, Mete Cihan3,
Recai Tunca4, Başak Kurt3, Alparslan Kadir
Devrim5 and Hasan Özen1
1Department of Pathology,
Faculty of Veterinary Medicine, Kafkas University, Kars, Turkey;
2Department of Pathology, Faculty of Veterinary Medicine,
Ondokuzmayıs University, Samsun, Turkey; 3Department of
Surgery, Faculty of Veterinary Medicine, Kafkas University, Kars,
Turkey; 4Department of Pathology, Faculty of Veterinary
Medicine, Adnan Menderes University, Aydın, Turkey; 5Department
of Biochemistry, Faculty of Veterinary Medicine, Kırıkkale
University, Kırıkkale, Turkey *Corresponding author:serpildag06@hotmail.com
Abstract
Total of 30 domestic stray cats (Felis
cattus) were investigated for the presence of different species of gastric
Helicobacter-like organisms (GHLO) by
immunohistochemistry and polymerase chain reaction (PCR) analysis. The severity
and distribution of pathologic changes in different regions of stomach were
assessed microscopically. GHLO were present in all areas of the stomach in 93.3%
cats investigated. Morphologically two different types of spiral bacteria were
recognized. In 53.3% cats H. felis
like organisms and in 76.7% cats H.
heilmannii like organisms were determined. Mixed presence of both bacteria
was seen in 43.3% cases. H. pylori was
not detected in any of the cats. Mild to severe gastritis were observed in 90.0%
cats. GHLO were present in fundus, corpus and pyloric antrum regions in similar
densities. The most striking histopathological changes were lymphocyte and
neutrophil infiltrations, fibrosis in the lamina propria, and lymphoid follicle
formation. There was no significant relationship between the degree of bacterial
density and the extent of histopathological changes. GHLOs were present on the
mucosal surface, in the lumen of gastric glands, and in the cytoplasm of
parietal cells. In conclusion, PCR and immunohistochemistry can be successfully
used in detection of GHLOs. The results of the study show also that
H. heilmannii and
H. felis are frequent agents in stray
cats, and hence suggest that these animals might be common reservoirs for these
microorganisms. However, the bacteria do not seem to be solely responsible for
gastritis observed in some stray cats.