1Veterinary
Drugs & Biologics Division, Animal and Plant Quarantine Agency,
Anyang, Gyeonggi-do 430-757, Republic of Korea; 2Department
of Physiology, College of Veterinary Medicine, Kyungpook National
University, Daegu 702-701, Republic of Korea *Corresponding author: leekwj@korea.kr (KJL); twkim@knu.ac.kr (TWK)
Abstract
The current study compares the performance and
evaluated the practicality of three rapid detection methods for β-lactamase
production among 48 veterinary bacterial isolates (16
S. aureus, 18 E. coli, and
14 S. typhimurium) in Korea.
Antimicrobial susceptibility was determined by broth microdilution method and β-lactamase
production was detected by iodometric, acidimetric, and chromogenic methods.
Seventy five percent of S.
aureus, 44% of
E. coli, and 35% of
S. typhimurium were resistant to
penicillin, ampicillin, and amoxicillin, respectively. Overall, the iodometric
test yielded the highest positive results (91.67%), followed by chromogenic test
(66.67%) and acidimetric test (47.92%). The sensitivities and specificities of
the three different tests varied for S. aureus, E.
coli, and
S. typhimurium isolates. Our results showed that these test methods
may applicable to monitor β-lactamase production among various microorganisms
and to predict β-lactam antibiotic resistance.
Key words:
Antimicrobial drug Resistance,
Korea,
MIC,
Veterinary isolate,
β-lactam detection, β-lactamases