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Comparison of Three Rapid Tests for the Detection of β-Lactamase Production among Bacterial Isolates
Jeong Woo Kang§,1,2, Seonhwa Kim§,1, Hae-Chul Park1, Yangho Jang1, Mamata Gurung1, Jae-Young Song1, Kwang-Jick Lee1,* and Tae-Wan Kim2,*
1Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do 430-757, Republic of Korea; 2Department of Physiology, College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Republic of Korea
*Corresponding author: (KJL); (TWK)


The current study compares the performance and evaluated the practicality of three rapid detection methods for β-lactamase production among 48 veterinary bacterial isolates (16 S. aureus, 18 E. coli, and 14 S. typhimurium) in Korea. Antimicrobial susceptibility was determined by broth microdilution method and β-lactamase production was detected by iodometric, acidimetric, and chromogenic methods. Seventy five percent of S. aureus, 44% of E. coli, and 35% of S. typhimurium were resistant to penicillin, ampicillin, and amoxicillin, respectively. Overall, the iodometric test yielded the highest positive results (91.67%), followed by chromogenic test (66.67%) and acidimetric test (47.92%). The sensitivities and specificities of the three different tests varied for S. aureus, E. coli, and S. typhimurium isolates. Our results showed that these test methods may applicable to monitor β-lactamase production among various microorganisms and to predict β-lactam antibiotic resistance.

Key words: Antimicrobial drug Resistance, Korea, MIC, Veterinary isolate, β-lactam detection, β-lactamases


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)