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Prevalence of High-Level Aminoglycoside Resistant Enterococci Isolated from Tibetan Pigs
YF Lan1, K Li1, H Zhang1, SC Huang1, MU Rehman1, LH Zhang1, HQ Luo1, L Wang1, ZQ Han1, M Shahzad1 and JK Li1,2*
1College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; 2Laboratory of Detection and Monitoring of Highland Animal Disease, Tibet Agriculture and Animal Husbandry College, Linzhi 860000 Tibet, People's Republic of China
*Corresponding author:


Enterococci are considered as important opportunistic pathogens causing different infections in farm animals. The main aim of the experiment was to describe the prevalence of high-level aminoglycoside resistant (HLAR) Enterococci isolated from Tibetan pigs. In current study, 51 samples were identified by polymerase chain reaction (PCR) followed by testing the high level streptomycin resistance (HLSR) and high level gentamicin resistance (HLGR) by Minimum Inhibitory Concentration (MIC). The aminoglycoside modifying enzyme (AME) genes aac(6’)-Ie-aph(2’)-Ia, aph(2)-Ic, aph(3)-IIIa, ant(4)-Ia, ant(6’)-Ia and ant(3’)-(9) in Enterococci were detected by PCR. Results showed that the prevalence of HLSR, HLGR and HLGR + HLSR was found to be 68.6, 76.5, and 39.2% respectively; and ant (4’)-Ia and aac(6’)-Ie-aph(2’)-Ia genes showed 62.7 and 70.6% prevalence, respectively; however, other AME genes were found all negative. In conclusion, the isolates of Enterococci from Tibetan pigs have produced high level resistance to aminoglycoside.

Key words: Antimicrobial resistance, Enterococcus, MIC, Polymerase chain reaction, Tibetan pigs


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)