One health links the health of humans with that of animals and the environment. Pseudomonas aeruginosa, a common nosocomial and opportunistic pathogen of humans and animals causing cystic fibrosis, dermatitis, soft tissue infections, gastrointestinal infections and mastitis etc., has been prevalent in many hospital environments throughout the world. In present the study, out of six hundred samples taken either directly or from close proximity of humans, bovines and equines keeping in view the one health concept of P. aeruginosa. All the samples were checked through conventional methods, API KIT, Biofilm detection, Colony PCR, sequencing and in vivo experiments for taxonomic, molecular and pathogenic characterization of P. aeruginosa isolates. Present study indicates the prevalence of virulent strains of P. aeruginosa in higher order of frequency of isolation i.e. 13.5, 9 and 7.5% in three different hospital environments including Allied Hospital (AH), District Head Quarter Hospital (DHQH) and Civil Veterinary Hospital (CVH) respectively. P. aeruginosa could be recovered from humans, bovines and equines throat swabs with or without symptoms (14.7%), wound pus (13.3%), surgical instruments (8.6%) and garden soil (3.3%). However, the most commonly prevalent and virulent isolates were confirmed as P. aeruginosa strain BTP1 (KU534099) and P. aeruginosa strain WPP1 (KU534100) and consequent phylogenetic analyses clustered them with the most narrowly related Pseudomonas species belonging to the famous P. aeruginosa PAO1 groups. Such a higher order prevalence of these strains in diverse environments and their physiological, genetic and pathogenic variation is quite alarming demanding special considerations from the concerned quarters for one health promotion in Pakistan.

Key words: 16SrRNA gene, Clinical environment, Multi drug resistance, Nosocomial infections, One health, Pseudomonas aeruginosa