Pathology and Phylogenetic Analysis of Capripoxvirus
in Naturally Infected Sheep Sheeppox Virus
Z Karapinar1*, F Ilhan 2, E
Dincer3 and S Yildirim4
1Faculty
of Veterinary Medicine, Department of Virology, YuzuncuYil
University, Van, Turkey; 2Faculty of Veterinary Medicine,
Department of Pathology, Balikesir University, Balikesir, Turkey;
3Research and Application Center, Mersin University
Advanced Technology Education, Mersin, Turkey; 4Faculty
of Veterinary Medicine, Department of Pathology, Ataturk University,
Erzurum, Turkey *Corresponding author: zeynepkarapinar@gmail.com
Abstract
Sheeppox and goatpox is a contagious viral
disease of sheep and goats characterized by fever, generalized papules or
nodules in skin and mucosal surface. DNA virus belonging to the genus
capripoxvirus. The disease with high mortality and morbidity causes significant
economic losses in small ruminants. In this study, 20 tissues from 8 sheep which
were considered having natural sheeppox based on the macroscopic and
histopathologic evaluation were further investigated by immunohistochemical (iNOS,
SP-A, HSP-70), and Polymerase Chain Reaction (PCR) method. Papules, typically
pox lesions, noticeable on the skin surfaces were examined macroscopically.
Immunohistochemically; iNOS and SP-A were most intensely stained whereas HSP-70
was low stained. PCR method was used for the detection of A29L gene of capripoxvirus.
Positive samples obtained from sheep were used for molecular characterization. A
phylogenetic analysis was performed using sequence of the partial A29L gene and
by comparing with reference sheeppox viruses isolates obtained from Gene Bank.
The results of the sequence analysis were similar among themselves, they were
found different (99-100% identity) from the other sheeppox viruses around the
world. This study provides firstly phylogenetic analysis of sheeppoxviruses from
Van province in Turkey.