West Nile Virus (WNV) antibody presence was studied in domestic horse (Equus caballus) and donkeys (Equus asinus) of various age and types owned by public in different regions of Turkey. In the study, serum samples were collected from 650 domestic horses and 234 domestic donkeys seeming to be in good health. In order to detect WNV antibody presence in these samples, commercial c-ELISA (Competitive Enzyme Linked Immunosorbent Assay) kit (ID Screen® West Nile Competition-IgG-ELISA kit, Montpellier, France) was used. At the end of the test, WNV seropositivity was detected at a level of 4.15% (27/650) for horse serum samples and 1.28% (3/234) for donkey serum samples. Seropositivity was found for horses in Aegean and Central Anatolia and for donkeys in Eastern Anatolia regions. WNV seropositivity was detected only for Native Anatolia type horse races. It was concluded that WNV presence continued its existence among equidae in our country.

Key words: Donkey, ELISA, Horse, Serology, West nile virus