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Serological Investigation of West Nile Virus Infection in Domestic Horses and Donkeys in Turkey
Kale Mehmet1,*, Gur Sibel2, Yapıcı Orhan3, Mamak Nuri4, Yavru Sibel3, Hasırcıoglu Sibel1, Bulut Oya3 and Gurcay Metin5
1Department of Virology, Mehmet Akif Ersoy University, Turkey; 2Department of Virology, Afyon Kocatepe University, Turkey; 3Department of Virology, Selçuk University, Turkey; 4Department of Internal Medicine, Mehmet Akif Ersoy University, Turkey; 5Department of Virology, Elazıg Veterinary Control Institute, Turkey
*Corresponding author:


West Nile Virus (WNV) antibody presence was studied in domestic horse (Equus caballus) and donkeys (Equus asinus) of various age and types owned by public in different regions of Turkey. In the study, serum samples were collected from 650 domestic horses and 234 domestic donkeys seeming to be in good health. In order to detect WNV antibody presence in these samples, commercial c-ELISA (Competitive Enzyme Linked Immunosorbent Assay) kit (ID Screen® West Nile Competition-IgG-ELISA kit, Montpellier, France) was used. At the end of the test, WNV seropositivity was detected at a level of 4.15% (27/650) for horse serum samples and 1.28% (3/234) for donkey serum samples. Seropositivity was found for horses in Aegean and Central Anatolia and for donkeys in Eastern Anatolia regions. WNV seropositivity was detected only for Native Anatolia type horse races. It was concluded that WNV presence continued its existence among equidae in our country.

Key words: Donkey, ELISA, Horse, Serology, West nile virus


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)