Molecular Characterization of Local Isolates of
Mycoplasma capricolum Sub
Specie Capripneumoniae in
Goats (Capra hircus) of
Khyber Pakhtunkhwa, Pakistan
Muhammad Kamal Shah1*, Umer Saddique1,
Shakoor Ahmad1, Aqib Iqbal2,Abid
Ali2, Waseem Shahzad3, Muhammad Sayyar Khan2,
Hamayun Khan1, Hanif Ur Rahman1, Said Sajjad
Ali Shah1 and Muhammad Israr4
1Department
of Animal Health, Faculty of Animal Husbandry & Veterinary Sciences,
the University of Agriculture, Peshawar, Pakistan; 2Institute
of Biotechnology & Genetic Engineering, University of Agriculture,
Peshawar; 3Veterinary Research Institute, Lahore,
Pakistan; 4Pakistan
Science Foundation, Islamabad, Pakistan *Corresponding author: kamaluaf560@gmail.com
Abstract
Caprine mycoplasmosis
is an important infectious respiratory complication lead to significant health
issue and causes heavy economic losses in small ruminant population throughout
the world. The study was designed to identify and characterize the pathogenic
member of mycoplasmacluster the
Mycoplasma capricolum sub specie
capripneumoniae (Mccp)isolated fromgoat suffering from respiratory syndrome in the natural outbreak. The study
was carried out during November, 2013 to April, 2015 in the Khyber Pakhtunkhwa
(KPK), Pakistan. A total of 825 samples from nasal discharge, tracheal swab,
pleural fluid and lung tissue were collected from goat clinically suspected for
Contagious Caprine Pleuro Pneumonia (CCPP). The samples taken in PPLO transport
media were cultured on modified Hay Flick media and incubated at 37°
C with 5% CO2 for 7-12 days. Out of total 267 (32.36%) were positive
for mycoplasma growthshowing mass
turbidity, whirling movement in culture broth and typical fried egg colonies in
agar media. The positive culture was identified through biochemical assay and
confirmed as Mccp through PCR by using
cluster and specie specific primers. Out of 267 positive samples 55 (20.59%)
were confirmed as Mycoplasmamycoides cluster and 23 (8.61%) were identified as
Mccp. Sequencing of the 16-S rRNA gene
blast search revealed 99% sequence homology with
Mccp compared with eight available
sequences at NCBI. The presence of Mccp
was for the first time reported in small ruminants of Khyber Pakhtunkhwa.
Key words:
Goat,
Hay Flick media,
Mycoplasma,
Pakistan,
PCR, Sequencing