Occurrence and Antibiotic Susceptibility of
Methicillin-Resistant
Staphylococcus aureus Recovered from Oropharynx of Live
Cockerels
Z Zaheer1*, I Hussain1, SU
Rahman1, T Younas1, I Zaheer2, G
Abbas1 and M Nasir3
1Institute of Microbiology;
2Department of Pathology, Faculty of Veterinary Science,
University of Agriculture, Faisalabad, Pakistan; 3Department
of Zoology, University of the Punjab, Lahore, Pakistan *Corresponding author:
zaytoonzaheer@gmail.com
Abstract
Methicillin-resistant Staphylococcus aureus (MRSA)
was recovered many times from raw poultry meat or carcasses; however, these were
predominantly human-associated strains. Hence, possible human involvement in
contamination of carcasses by slaughterhouse workers and other human handlers
may not be overlooked in such cases. During this study, efforts were focused on
isolation of MRSA from oropharynx of live poultry. Samples were collected from
oropharynx of 50 live cockerels. A total of 25
Staphylococcus aureus isolates were
identified. Only four isolates produced glistening, convex and mucoid colonies
of MRSA on selective media. These isolates were further confirmed for
methicillin resistance through latex agglutination test and produced catalase
and coagulase, β-hemolysis on blood agar. Out of 25
Staphylococcus aureus isolates, 16% isolates were identified as
MRSA. Antibiotic susceptibility profile of MRSA isolates indicated 100%
sensitivity against vancomycin and linezolid whereas 100% resistance was
recorded against oxacillin, cefoxitin and penicillin. However, 50% of the MRSA
isolates were sensitive to levofloxacin and trimethoprim/ sulphamethoxazole and
25% isolates displayed resistance to tetracycline.