Investigation of Staphylococcus aureus Enterotoxins A to E Via
Real-Time PCR from Various Food Samples in Turkey
Mehmet Demirci1*,
Ferruh Tombul2, Akın Yiğin3 and Serap Kılıç
Altun4
1University of Istanbul, Cerrahpaşa Medical
Faculty, Medical Microbiology, 34089, Istanbul–Turkey; 2Republic
of Turkey, Ministry of Food, Agriculture and Livestock, Istanbul
Food Control Biogenetic laboratory, Istanbul-Turkey; 3University
of Harran, Faculty of Veterinary Medicine, Department of Genetic,
63000, Şanlıurfa – Turkey; 4University of Harran, Faculty
of Veterinary Medicine, Department of Food Hygiene and Technology,
63000, Şanlıurfa – Turkey
*Corresponding author: demircimehmet@hotmail.com
Abstract
The
aim of this study was to investigate the Staphylococcal enterotoxin (SE) and
directly detect the five classical SEA, SEB, SEC, SED and SEE gene in
Staphylococcus aureus strains from
different food samples by real-time PCR.
We studied totally 3650 different food samples such as milk
and dairy products, meat and meat products, poultry and eggs, canned food,
coffee, cocoa and derived products, honey, confectionery and bakery,
ready-to-eat foods, beverages in food control laboratory. We found that a total
of 36 (0.98%) S. aureus strains were isolated and only 14 (0.38%) out of 36
S. aureus strains were found to be
enterotoxingenic. Milk samples were found to be most contaminant among the
products. The most prevalent SE types were SEA 7 (19.4%). Commercial EIA kit
results were used to compare with
the real-time PCR results. SE results were found to be same with these
two methods. This study showed that prevalence and type of the staphylococcal
enterotoxin may vary from food to food. It is important to know this data to
prevent outbreaks. Additionally, automated DNA isolation and real-time PCR
methods can be performed to direct enterotoxin gene detection rapidly and
reliably. This technique can be also used for food safety and clinical diagnosis
applications.
Key words:
Direct detection,
Enterotoxin genes,
Food,
Real-time PCR,
Staphylococcus aureus