1Institute of Animal
Population Health, College of Animal Science and Technology, JiangXi
Agricultural University, N.O. 1101, Zhimin Avenue, Nanchang Economic
and Technological Development District Nanchang 330045, P.R. China;
2Clinical Veterinary Laboratory, College of Animal
Science and Technology, Guangxi University, Nanning, Guangxi 530005,
People’s Republic of China *Corresponding author: PingLiujx@163.com;
229208133@qq.com
Abstract
To evaluate the microbial population in milk from
dairy cows with mastitis in Guangxi Province, China, 11 fresh milk samples were
collected from cows with mastitis at a farm in the province with 1000 Holstein
dairy cows. A CMT was performed on the milk samples, and they were classified by
parity: A (1th), B (2nd), and C (3rd). The
microbial community was analyzed via deep DNA sequencing of the bacterial 16S
rRNA genes using the Illumina MiSeq platform. The results revealed that there
were many bacteria and fungi present in the milk samples. Ten
bacterial phyla (Acidobacteria, Actinobacteria, Bacteria-unclassified,
Bacteroidetes, Candidate-division-TM7, Cyanobacteria, Firmicutes, Fusobacteria,
Proteobacteria, and Tenericutes) were identified. Firmicutes was the
predominant phylum, followed by Tenericutes. The fungi found in the
samples belonged to 2 phyla (Ascomycota and Basidiomycota). At the genus level,
the most abundant bacterial operational taxonomic units (OTUs) were
Enterococcus and Mycoplasma.
The most abundant fungal genus was
Malassezia, followed by Agaricales-unclassified. The data indicated that the
predominant phylum in the milk samples was associated with climate, antibiotic
resistance, and parity. In this study, we provide a theoretical foundation for
research on the prevention of mastitis as well as the selection of medicine for
mastitis treatment.