Antibiotic Resistance of
Escherichia coli in Free-Ranging Yaks
(Bos grunniens) from Tibetan Plateau, China
Mujeeb Ur Rehman1,Hui Zhang1,Muhammad Kashif Iqbal1,
Fazul Nabi1,
Shucheng Huang1, Yanfang Lan1, Khalid
Mehmood1,3, Houqiang Luo1 and Jiakui Li1,2*
1College of Veterinary Medicine, Huazhong
Agricultural University, Wuhan 430070, P.R. China; 2Laboratory
of Detection and Monitoring of Highland Animal Diseases, Tibet
Agricultural and Animal Husbandry College, Linzhi, 860000, Tibet,
P.R. China; 3College of Veterinary and Animal Sciences,
Islamia University of Bahawalpur, Pakistan;
*Corresponding author:
lijk210@sina.com
Abstract
In this study, we collected 565 fecal samples
from free-range Tibetan yaks to examine whether these animals can serve as a
reservoir for pathogenic and
antimicrobial-resistant Escherichia
coli. The antimicrobial sensitivity testing of each resulting
isolate was evaluated according to the
disk diffusion method,whereas PCR analysis was performed for detection
of resistance and virulence genes. Additionally, strains containing
extended-spectrum beta-lactamases (ESBLs) genes were initially identified via
the double-disk synergy method and confirmed by PCR analysis. Of the 488
E. coli isolates examined,
approximately 31.1% were multidrug resistant. OmpA (30.2%) and etrA (23.1%), and blaCTX-M (27.6%) and blaTEM
(14.4%) were the mainly prevalent virulence and drug resistance genes,
respectively. Notably, we detected the CTX-M type but not the SHV type among the
ESBL-producing strains and there was a significant association between
resistance and virulence genes for aac(3)-lla,blaTEM/ompA,blaCTX-M/etrA,and cmlA/cnf1
(P<0.05). Lastly, the majority of the strains belonged to phylogroup A (72.7%).
This is the first report of the occurrence of
pathogenic and antimicrobial-resistant
ESBL-producing E. coli strains
in free-range Tibetan yaks.
Key words:
Antimicrobial-resistant, Disk
diffusionmethod, Double-disk synergy method, Escherichia coli