Recently the frequent use of different synthetic compounds like pesticides, herbicides, arsenic, insecticides and fertilizers had led to contamination of environment. Therefore, the current experimental study was conducted to ascertain the concurrent impacts of sodium arsenate and diammonium phosphate in commercial layer. For this purpose a total of 90, day old Lohmann Selected White leghorn chicks of similar weight and sex were procured and kept into six groups. Sodium arsenate and DAP were administered to experimental birds in groups (B-F) alone and in different combination. Group A was kept as control group. Various clinical and behavioral alterations such as watery droppings, dullness, gasping, depression, tremors, anemic comb and wattle were evident in birds. The body weight and relative weight of different organs was reduced significantly. Extensive fatty infiltration, pyknosis of hepatocyte and coagulative necrosis in liver, increased urinary space, necrosis of tubular epithelial cells and congestion in kidneys was observed. The total erythrocytes counts, hemoglobin concentration, hematocrit, mean corpuscular volume and mean corpuscular hemoglobin concentration were significantly reduced. The plasma proteins, serum total proteins, albumin and globulins levels were significantly decreased while serum creatinine and different enzymes were significantly increased in birds. The incidence of erythrocyte with micronucleus, blebbed nucleus and binucleated erythrocyte was significantly increased at different experimental days. In conclusion the arsenic (25 mg/kg bw) and DAP (10%) alone and in combinations (15 mg/kg bw arsenic +6% DAP) were the most toxic to birds.

Key words: Arsenic, Birds, Diammonium phosphate, Hemato-bichemistry, Histopathology