Expression of NKCC2, NCC and Na+-K+-ATPase
Genes Encoding during Acute Renal Failure in Dogs
Hui Zhang1,§, Zhenyu Chang1,2,§,
Meixian Bao1, Mujeeb Ur Rehman1,
Khalid Mehmood1,3, Fazul Nabi1,4, Xiaoxing Wu1, Xinxin Tian1,
Xiaodan Yuan1, Muhammad Ijaz5 and Donghai Zhou1,*
1College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, People's Republic of China;
2Key laboratory of clinical veterinary medicine in Tibet,
Tibet Agriculture and Animal Husbandry College, Linzhi 860000 Tibet,
China; 3University College of Veterinary and Animal
Sciences, Islamia University of Bahawalpur-63100, Pakistan; 4Lasbela University of Agriculture, Water and Marine Science Uthal, Pakistan;
5Department of Clinical Medicine & Surgery, University
of Veterinary and Animal Sciences Lahore-54000, Pakistan *Corresponding author:
bigdefoot@163.com
Abstract
Acute renal failure is a condition in which
kidneys are unable to eliminate all the waste from the body. Renal failure
results in acid-base imbalance and electrolyte homeostasis. Na+-K+-2Cl-
Cotransporter (NKCC2), Na+-Cl- Cotransporter (NCC) and Na+-K+-ATPase
Cotransporter (Na+-K+-ATPase) are the important water and
sodium channels proteins in the kidney. The aim of this study was to determine
the gene expression level in the kidney of dogs suffering with the acute renal
failure. A total four dogs with acute renal failure and four healthy
dogs were used in this study. The biochemical analysis was done via semi-automatic biochemical machine,
while the urine and urinary sediment examination was
observed via microscopic study. The acute renal failure was diagnosed and
confirmed via necropsy and histopathological examinations and finally the protein expression was analyzed by
immunohistochemistry. Results showed that
the concentration of sodium, potassium, creatinine
and urea nitrogen were significantly different from control group. The level of pH and specific gravity (SG) were
decreased with urobilinogen (URO) and were increased during acute renal failure.
The urinary sedimentation results revealed the presence of RBCs and WBCs in
urine samples. The kidney volume was increased with renal capsule due to
hyperplasia and renal pelvis expansion. The hydronephrosis was also observed
along with hemorrhages, shrinkage, necrosis, and inflammation in acute renal
failure groups by histopathology. The
immunohistochemical results revealed that the
NKCC2, NCC and Na+-K+-ATPase
were highly expressed in acute renal failure dogs. Therefore, it was concluded
that sodium channels proteins possibly play
an important role in dog’s acute renal failure cases.