This study was conducted to compare two commercially available point-of-care enzyme-linked immunosorbent assay kits (TiterCHEK^® and ImmunoComb^®) for the detection of antibodies against CPV and CDV. Dogs were clinically healthy, and included 22 (21%) beagles and 83 (79%) Korean native bobtailed dogs admitted to an animal shelter. Of the 105 dogs, 89 (84.76%) were found positive for CPV protective antibody titers (PATs) by HI assay and 3 (2.85%) were identified as positive for CDV PATs by SN assay. The CPV PAT was significantly higher (Pearson correlation coefficient=0.297, P=0.002) in adult dogs (≥1 year) than that in young dogs (<1 year). For an accurate identification of CPV and CDV PATs, although not statistically significant, the specificity (100% for both CPV and CDV) and positive predictive values (PPV, 100% for both CPV and CDV) of the TiterCHEK were higher than those of the ImmunoComb (specificities for CPV and CDV, 81.3% and 97.1%; PPV for CPV and CDV, 81.3% and 97.1%, respectively). The TiterCHEK had fewer false-positive results than the ImmunoComb. To the best of our knowledge, this is the first study that shows compare sensitivity and specificity between the TiterCHEK^® and ImmunoComb^® kits, using CPV HI and CDV SN as reference tests.

Key words: Antibody, Canine distemper virus, Canine parvovirus, Dog, ELISA